Jie Li, PhD. Professor, Division of Chemical Biology, School of Chemistry and Chemical Engineering, Nanjing University; Joint Professor of Chemistry and Biomedicine Innovation Center, Nanjing University.

T-cell Chemical Biology: Cross-cell labeling; Clone-specific T-cell manipulation; Metalloimmunology

Liu Z†, Li J†*, Chen M†, Wu M, Shi Y, Li W, Teijaro J, Wu P*, “Detecting tumor antigen-specific T cells via interaction-dependent fucosyl-biotinylation”, Cell, 2020, 183, 1117–1133.

Wu Y†, Deng T†, Song L†, Zhu W, Zhong D, Jiao J, Li W, Zhong L, Tian T, Dong L*, Li J*, “Clone-Resolved Chemical Depletion of  T cells via Cellular Proximity Chemistry”, Angew. Chem. Int. Ed., 2025, 64, e202425628.

Zhao Z*, Wu X, Zhang T, Zhou M, Liu S, Yang R, Li J*, “Evaluation of Multispecific Drugs Based on Patient-Derived Immunocompetent Tumor Organoids”, ChemBioChem, 2025, 26,3, e202400731.

Pan Y†, Xue Q†, Yang Y†, Shi T, Wang H, Song X, Luo Y, Liu W, Ren S, Cai Y, Nie Y, Song Z, Liu B, Li J*, Wei J*, “Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement”, Cell Rep. Med., 2024, 5, 6, 101590.

Xue Q†, Yu W†, Li J*, He C, Guo Z*, “Revealing the nature of Pt-based immunotherapy through the lens of neoantigens in cancer”, Sci. Bull., 2024, 69, 15, 2314-2318.

Zhao Z†, Zhang S†, Jiang N, Zhu W, Liu S, Yu W, Bai Y, Zhang Y, Guo H, Guo Z*, Yang R*, Li J*, “Patient-derived Immunocompetent Tumor Organoids A Platform for Chemotherapy Evaluation in the Context of T-cell Recognition”, Angew. Chem. Int. Ed., 2024, 63, e20231761.

Zhang S†, Song D†, Yu W†, Li J†, Wang X, Li Y, Zhao Z, Xue Q, Zhao J, Li J*, Guo Z*, “Combining cisplatin and a STING agonist into one molecule for cancer metalloimmunotherapy”, Natl. Sci. Rev.,2024, 11, 1, nwae020.

Yu W, Xu X, Liu L, Yang Y, Fan H*, Li J*, Guo Z, “Metal-based interventions in the immune “molecular machinery””. Sci. Sin. Chim.,2024, 54, doi: 10.1360/SSC-2023-0256.

Liu K, Zhang Z, Liu R, Li J*, Jiang D, Pan R*, “Click-Chemistry-Enabled Nanopipettes for the Capture and Dynamic Analysis of a Single Mitochondrion inside One Living Cell”, Angew. Chem.Int. Ed.,2023,62, e20230305.

Yu W†, Zhao X†, Jalloh A†, Li Y, Zhao Y, Dinner B, Yang Y, Ouyang S, Tian T, Zhao Z, Yang R, Chen M, Lauvau G, Guo Z*, Wu P*, Li J*,“Chemoenzymatic Measurement of LacNAc in Single-Cell Multiomics Reveals It as a Cell-Surface Indicator of Glycolytic Activity of CD8⁺ T Cells”,J. Am. Chem. Soc.,2023, 145, 23, 12701-12716.

Yang Y†, Song Z†, Tian T†, Zhao Z, Chen J, Hu J, Jiang X, Yang G, Xue Q, Zhao X, Sha W, Yang Y*, Li J*, “Trimming Crystallizable Fragment (Fc) Glycans Enables the Direct Enzymatic Transfer of Biomacromolecules to Antibodies as Therapeutics”, Angew. Chem. Int. Ed.,2023, 62, e202308174.

Qiu S†, Li W†, Deng T†, Bi A, Yang Y, Jiang X, Li J*, “Ru(bpy)₃²⁺-Enabled Cell-Surface Photocatalytic Proximity Labeling toward More Efficient Capture of Physically Interacting Cells”, Angew. Chem. Int. Ed.,2023, 62, e202303014.

Wu Q, Xue Q, Li J, Zheng Q, Zhao X, Li W, Sun S, Sha W, Yang Y, Yang Y, Li J*, “Identification of Styryl Sulfonyl Fluoride (SSF) as a Near Perfect Michael Acceptor for Diversified Protein Bioconjugations”, CCS Chem.,2023, 5, 2251-2263.

Qiu S†, Zhao Z†, Wu M†, Xue Q, Yang Y, Ouyang S, Li W, Zhong L, Wang W, Yang R, Wu P*, Li J*, “Use of Intercellular Proximity Labeling to Quantify and Decipher Cell-cell Interactions Directed by Diversified Molecular Pairs”, Sci. Adv., 2022, 8, eadd233.

Zhao X†, Sun S†, Yu W†, Zhu W, Zhao Z, Zhou Y, Ding X, Fang N, Yang R, Li J*, “Improved ClickTags Enable Live-cell Barcoding for Highly Multiplexed Single-cell Sequencing”, RSC Chem. Biol., 2022, 3, 1052–1060.

Liu H†, Luo H†, Xue Q, Qin S, Qiu S, Liu S, Lin J, Li J*, Chen P*, “Antigen-specific T cell detection via photocatalytic proximity cell labeling (PhoXCELL)”, J. Am. Chem. Soc., 2022, 144, 5517−5526.

Kiessling L, Chen P, Wang J, Li J*, “Fighting the Coronavirus Outbreak”, ACS Chem. Biol., 2020, 15, 4, 799–801.

Zhu W†, Wu X†, Zhao Z*, Zhou M, Zhang T, Zhao D, Jiang N, Yang X, Guo H, Fang N, Li J, Yang R*, Tian T*, “In Vivo Time-Resolved Single-Cell RNA-Seq Reveals Chemotherapy-Induced Transcriptional Dynamics in Tumor Infiltrating Lymphocytes”, Anal. Chem., 2025, 97,6, 3438–3448.

Li J†, Chen M†, Liu Z, Zhang L, Felding BH, Moremen KW, Lauvau G., Abadier M, Ley K, Wu P, “A Single-Step Chemoenzymatic Reaction for the Construction of Antibody–Cell Conjugates”, ACS Cent. Sci., 2018, 4, 1633–1641.

Liu Z†, Li J†, Li S†, Li G, Sharpless KB, Wu P, “SuFEx Click Chemistry Enabled Late-Stage Drug Functionalization”, J. Am. Chem. Soc., 2018, 140, 2919–2925.

Li J, Chen P, “Development and Application of Bond Cleavage Reactions in Bioorthogonal Chemistry”, Nat. Chem. Biol., 2016, 12, 129-137.

Zhang G†, Li J†, Xie R, Fan X, Liu Y, Zheng S, Ge Y, Chen P, “Bioorthogonal Chemical Activation of Kinases in Living Systems”, ACS Cent. Sci., 2016, 2, 325–331.

Li J, Yu J, Zhao J, Wang J, Zheng S, Lin S, Chen L, Yang M, Jia S, Zhang X, Chen P, “Palladium-triggered Deprotection Chemistry for Protein Activation in Living Cells”, Nature Chem., 2014, 6, 352-361.

Li J†, Jia S†, Chen P, “Diels–Alder Reaction-triggered Bioorthogonal Protein Decaging in Living Cells”, Nat. Chem. Biol., 2014, 10, 1003-1005.

Zheng S, Zhang G, Li J, Chen P, “Monitoring Endocytic Trafficking of Anthrax Lethal Factor by Precise and Quantitative Protein Labeling”, Angew. Chem. Int. Ed., 2014, 53, 6449-6453.

Li J, Lin S, Wang J, Ja S, Yang M, Hao Z, Zhang X, Chen P, “Ligand-free Palladium-mediated Site Specific Protein Labeling Inside Gram-negative Bacterial Pathogens”, J. Am. Chem. Soc., 2013, 135, 7330-7338.