李劼，1988年11月生，江苏泰兴人，至诚特聘教授，博士生导师 。

2010年本科毕业于武汉大学化学与分子科学学院化学基地班；

2015年毕业于北京大学化学院化学生物学系，获博士学位；

2018年加入南京大学化学化工学院化学生物学学科；

2019年加入南京大学化学和生物医药创新研究院，入选江苏特聘教授；

2020年入选国家级青年人才；

2024年入选江苏省首届攀登项目；

2025年入选基金委青年科学基金项目A类（原国家杰青），卓越研究群体项目B类（骨干，原基础科学中心）。

课题组目前主要聚焦T细胞化学免疫学研究，致力于解码T细胞抗原识别的分子规律与命运重塑的关系，并通过多种化学手段进行调控，以期开发新的细胞疗法和大分子偶联药物。

欢迎直博生、硕士生、硕博生、博士后加入！

2015年到2018年在美国Scripps研究所（Scripps Research Institute）化学系及分子药学系从事博士后研究工作，合作导师为Barry Sharpless教授（两次诺贝尔化学奖得主）及Peng Wu 教授。

T细胞化学免疫学：1）免疫识别的化学解码与重塑； 2）免疫微环境的化学单细胞组学； 3）金属元素的免疫功能；4）新型免疫药物或细胞疗法的开发。

通讯文章

Liu Z†, Li J†*, Chen M†, Wu M, Shi Y, Li W, Teijaro J, Wu P*, “Detecting tumor antigen-specific T cells via interaction-dependent fucosyl-biotinylation”, Cell, 2020, 183, 1117–1133.

Wang Z†, Xiang F†, Liao X†, Wu Q, Jiao J, Bi A, Liu S, Wang D, Wang M, Guo Z*, Li J*, Inert Complexes Unlock Ligand-Accelerated Transition-Metal Catalysis on Proteins, Angew. Chem. Int. Ed., 2026, e22057.

Deng T†; Jiang X†; Zhao Z; Zhao X; Bi A; Ouyang S; Sun S; Qiu S; Fan M; Xue Q; Yu W; Yang Y; Li W; Wu Y; Huang X; Zhong D; Qin S; Zhu W; Li J*, CINTER-seq: Chemical profiling reveals interaction-dependent cell landscapes and gene signatures in vivo, Immunity, 2025, 58, 2336-52.

Wu Y†, Deng T†, Song L†, Zhu W, Zhong D, Jiao J, Li W, Zhong L, Tian T, Dong L*, Li J*, “Clone-Resolved Chemical Depletion of T cells via Cellular Proximity Chemistry”, Angew. Chem. Int. Ed., 2025, 64, e202425628.

Xue Q†, Peng J†, Dai W†, Wu Q, Jiao J, Hu Y, Sha W, Yang Y, Yu W, Liu S, Xu T*, Li J*, A biparatopic HER2-targeting ADC constructed via site-specific glycan conjugation exhibits superior stability, safety, and efficacy, RSC Chem. Bio., 2025, 6, 1284-96.

Pan Y†, Xue Q†, Yang Y†, Shi T, Wang H, Song X, Luo Y, Liu W, Ren S, Cai Y, Nie Y, Song Z, Liu B, Li J*, Wei J*, “Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement”, Cell Rep. Med., 2024, 5, 6, 101590.

Xue Q†, Yu W†, Li J*, He C, Guo Z*, “Revealing the nature of Pt-based immunotherapy through the lens of neoantigens in cancer”, Sci. Bull., 2024, 69, 15, 2314-2318.

Zhao Z†, Zhang S†, Jiang N, Zhu W, Liu S, Yu W, Bai Y, Zhang Y, Guo H, Guo Z*, Yang R*, Li J*, “Patient-derived Immunocompetent Tumor Organoids A Platform for Chemotherapy Evaluation in the Context of T-cell Recognition”, Angew. Chem. Int. Ed., 2024, 63, e20231761.

Zhang S†, Song D†, Yu W†, Li J†, Wang X, Li Y, Zhao Z, Xue Q, Zhao J, Li J*, Guo Z*, “Combining cisplatin and a STING agonist into one molecule for cancer metalloimmunotherapy”, Natl. Sci. Rev.,2024, 11, 1, nwae020.

Yu W, Xu X, Liu L, Yang Y, Fan H*, Li J*, Guo Z, “Metal-based interventions in the immune “molecular machinery””. Sci. Sin. Chim.,2024, 54, doi: 10.1360/SSC-2023-0256.

Liu K, Zhang Z, Liu R, Li J*, Jiang D, Pan R*, “Click-Chemistry-Enabled Nanopipettes for the Capture and Dynamic Analysis of a Single Mitochondrion inside One Living Cell”, Angew. Chem.Int. Ed.,2023,62, e20230305.

Yu W†, Zhao X†, Jalloh A†, Li Y, Zhao Y, Dinner B, Yang Y, Ouyang S, Tian T, Zhao Z, Yang R, Chen M, Lauvau G, Guo Z*, Wu P*, Li J*, “Chemoenzymatic Measurement of LacNAc in Single-Cell Multiomics Reveals It as a Cell-Surface Indicator of Glycolytic Activity of CD8⁺T Cells”, J. Am. Chem. Soc.,2023, 145, 23, 12701-12716.

Yang Y†, Song Z†, Tian T†, Zhao Z, Chen J, Hu J, Jiang X, Yang G, Xue Q, Zhao X, Sha W, Yang Y*, Li J*, “Trimming Crystallizable Fragment (Fc) Glycans Enables the Direct Enzymatic Transfer of Biomacromolecules to Antibodies as Therapeutics”, Angew. Chem. Int. Ed.,2023, 62, e202308174.

Qiu S†, Li W†, Deng T†, Bi A, Yang Y, Jiang X, Li J*, “Ru(bpy)₃²⁺-Enabled Cell-Surface Photocatalytic Proximity Labeling toward More Efficient Capture of Physically Interacting Cells”, Angew. Chem. Int. Ed.,2023, 62, e202303014.

 Wu Q, Xue Q, Li J, Zheng Q, Zhao X, Li W, Sun S, Sha W, Yang Y, Yang Y, Li J*, “Identification of Styryl Sulfonyl Fluoride (SSF) as a Near Perfect Michael Acceptor for Diversified Protein Bioconjugations”, CCS Chem.,2023, 5, 2251-2263.

Qiu S†, Zhao Z†, Wu M†, Xue Q, Yang Y, Ouyang S, Li W, Zhong L, Wang W, Yang R, Wu P*, Li J*, “Use of Intercellular Proximity Labeling to Quantify and Decipher Cell-cell Interactions Directed by Diversified Molecular Pairs”, Sci. Adv., 2022, 8, eadd233.

Zhao X†, Sun S†, Yu W†, Zhu W, Zhao Z, Zhou Y, Ding X, Fang N, Yang R, Li J*, “Improved ClickTags Enable Live-cell Barcoding for Highly Multiplexed Single-cell Sequencing”, RSC Chem. Biol., 2022, 3, 1052–1060.

Liu H†, Luo H†, Xue Q, Qin S, Qiu S, Liu S, Lin J, Li J*, Chen P*, “Antigen-specific T cell detection via photocatalytic proximity cell labeling (PhoXCELL)”, J. Am. Chem. Soc., 2022, 144, 5517−5526.

Kiessling L, Chen P, Wang J, Li J*, “Fighting the Coronavirus Outbreak”, ACS Chem. Biol., 2020, 15, 4, 799–801.

Before NJU-代表文献

Li J†, Chen M†, Liu Z, Zhang L, Felding BH, Moremen KW, Lauvau G., Abadier M, Ley K, Wu P, “A Single-Step Chemoenzymatic Reaction for the Construction of Antibody–Cell Conjugates”, ACS Cent. Sci., 2018, 4, 1633–1641.

Liu Z†, Li J†, Li S†, Li G, Sharpless KB, Wu P, “SuFEx Click Chemistry Enabled Late-Stage Drug Functionalization”, J. Am. Chem. Soc., 2018, 140, 2919–2925.

Li J, Chen P, “Development and Application of Bond Cleavage Reactions in Bioorthogonal Chemistry”, Nat. Chem. Biol., 2016, 12, 129-137. 

Zhang G†, Li J†, Xie R, Fan X, Liu Y, Zheng S, Ge Y, Chen P, “Bioorthogonal Chemical Activation of Kinases in Living Systems”, ACS Cent. Sci., 2016, 2, 325–331.

Li J, Yu J, Zhao J, Wang J, Zheng S, Lin S, Chen L, Yang M, Jia S, Zhang X, Chen P, “Palladium-triggered Deprotection Chemistry for Protein Activation in Living Cells”, Nature Chem., 2014, 6, 352-361.

Li J†, Jia S†, Chen P, “Diels–Alder Reaction-triggered Bioorthogonal Protein Decaging in Living Cells”, Nat. Chem. Biol., 2014, 10, 1003-1005.

Li J, Lin S, Wang J, Ja S, Yang M, Hao Z, Zhang X, Chen P, “Ligand-free Palladium-mediated Site-Specific Protein Labeling Inside Gram-negative Bacterial Pathogens”, J. Am. Chem. Soc., 2013, 135, 7330-7338.

《疾病中的免疫武库》，周五第7-8节（秋季学期），鼓楼校区新教-101。

《化学生物学导论》，周一3-4节，仙林校区教学楼仙I-315。

《疾病中的免疫武库》：考核形式为小组汇报和课程作业。

《化学生物学导论》：总成绩：期中考试（开卷+闭卷）20%，文献汇报20%，平时成绩20%，期末考试（开卷+闭卷）40%。

《医学免疫学》、《工作细胞》、《How the Immune System Works》、《癌症、免疫与治愈》、《传染病与人类历史》、《Advanced Chemical Biology》、《Introduction to Bioorganic Chemistry and Chemical Biology》等。

2022年于紫清湖