Chen Yunlong
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Office Address:
E-mail: ylc@nju.edu.cn
Group Page: https://sklac.nju.edu.cn/hxju_en/
Personal Profile
Yunlong Chen, male, born in 1988, associate professor and master supervisor of School of chemistry, Nanjing University.
He is mainly engaged in the methodological research of in situ analysis of cellular glycosyl related biomolecules. He has developed a series of new methods, such as detection of cell surface glycosyl density, simultaneous detection of multi-component glycosyl, in situ imaging of protein-specific glycosyl, dynamic monitoring of intracellular glycosylation process, screening and regeneration analysis of cell surface glycolipid subclasses. At present, he focuses on:
1) Research on in-situ quantitative measurement of cellular glycoconjugates and methods for exploring their functions;
2) Research on in-situ measurement methods for in vivo glycoconjugates and their disease diagnosis strategies;
3) Research on in situ identification, intervention, and regulation methods for multiple glycoconjugates in vivo, as well as strategies for disease treatment and efficacy monitoring.
He has published 45 papers in journals including J. Am. Chem. Soc., Nat. Commun., Angew. Chem. Int. Ed..
ORCID: 0000-0002-3775-3028
ResearcherID：A-5563-2014
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Work Experience

2006-2010 School of Chemistry and Chemical Engineering, Nanjing University, Undergraduate;

2010-2015 School of Chemistry and Chemical Engineering, Nanjing University, PhD Candidate;

2015-2018 School of Chemistry and Chemical Engineering, Nanjing University, Assistant/Associate Research Fellow;

2018-2019 University of Oxford, Visiting Scholar;

2019-Now  School of Chemistry, Nanjing University, Associate Professor.

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Research

1) Research on in-situ quantitative measurement of cellular glycoconjugates and methods for exploring their functions;
2) Research on in-situ measurement methods for in vivo glycoconjugates and their disease diagnosis strategies;
3) Research on in situ identification, intervention, and regulation methods for multiple glycoconjugates in vivo, as well as strategies for disease treatment and efficacy monitoring.
Publications

************************************************2026***********************************************

45. Yi Ren,Yuexuan Meng, Huipu Liu, Guangming Li, Jiahui Sun, Jun Zhou, Jie Wu, Ying Liu, Lin Ding, Yunlong Chen*, Huangxian Ju*, Identification and quantification of intracellular N-acetylglucosamine modified glycoRNAs, Nature Communications, 2026, 10.1038/s41467-026-72909-4.

44. Yuanjiao Yang, Yuru Wang, Xuemei Li, Chaoyi Yang, Yuhui Yang, Yanyun Fang, Lin Ding, Yunlong Chen*, Huangxian Ju*, Real time in vivo efficacy monitoring of desialylation and perforation enhanced immunotherapy of cancer, BMEMat, 2026, 4, e70079.

43. Wencheng Xiao, Hang Ao, Nilin Wu, Jun Sun, Wenrui Hu, Yunlong Chen, Jie Wu*, Huangxian Ju*, Hemin-DNA switch-assisted microfluidic chemiluminescence immunoimaging for rapid and quantitative screening of specific hybridomas, Analytical Chemistry, 2026, 98(3), 2541–2549.

42. Qian Yu, Jun Sun, Yuan Chen, Chao Zhao, Yunlong Chen, Jie Wu*, Siqi Yu, Hong Liu, Huangxian Ju*, A daily-durable wearable sweat biosensor with robust reticular conductive biogel interface, Biosensors and Bioelectronics, 2026, 296, 118312.

************************************************2025***********************************************

41. Junjie Hu*, Fei Liu, Yunlong Chen, Xinxin Xie, Huangxian Ju*, A homogeneous mass-encoded strategy for mass spectrometric biosensing of multiplex proteins, Analytica Chimica Acta, 2025, 1364, 344219.

40. Chaoyi Yang, Yuexuan Meng, Ying An, Jing Jia, Yuru Wang, Guangming Li, Yiran Li, Shan Wu, Chengyao Geng, Yunlong Chen*, Huangxian Ju*, In Situ and Real-Time Multi-Modality Imaging Guided Orderly Triple-Therapy of Tumors with a Multifunctional Nanodrug, Advanced Science, 2025, 12, 202501048.

39. Qian Yu, Jie Wu, Wencheng Xiao, Chao Zhao, Weiqi Zhang, Yunlong Chen, Ying Liu, Hong Liu, Jun Zhou, Lin Ding, Huangxian Ju*, An integrated, wearable paper microfluidic array device for multicomponent electrochemical sweat sensing, Cell Reports Physical Science, 2025, 6(1), 102389.

38. Shan Wu, Yuru Wang, Yuhui Yang, Chaoyi Yang, Ayidana Jiensi, Chengyao Geng, Huangxian Ju*, Yunlong Chen*, In Situ and In Vivo Evaluation of Multiplex Protein-Specific Glycosylation of Tumors with a Dual-SERS Encoding Strategy, Analytical Chemistry, 2025, 97(1), 936–944.

************************************************2024***********************************************

37. Huipu Liu, Xuemei Li, Yi Ren, Yuanjiao Yang, Yunlong Chen*, Huangxian Ju*, In Situ Visualization of RNA-Specific Sialylation on Living Cell Membranes to Explore N-Glycosylation Sites, Journal of the American Chemical Society, 2024, 146(12), 8780–8786.

36. Yuhui Yang, Yiran Li, CaixiaWang, Yuru Wang, Yi Ren, Jie Wu, Huangxian Ju*, Yunlong Chen*, Ultra-Galactocation to Sialic Acid on Tumor Cells with A Penta-Functional Dendritic Probe for Enhanced Immuno-Killing, Angewandte Chemie-International Edition, 2024, 63(20), e202319849.

35. Yuru Wang, Shan Wu, Yuanjiao Yang, Yuhui Yang, Huipu Liu, Yunlong Chen*, Huangxian Ju*, In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen–cell interactions, Chemical Science, 2024, 15(11), 3901–3906.

34. Yiran Li, Haiqi Wang, Yunlong Chen, Lin Ding*, Huangxian Ju*, In Situ Glycan Analysis and Editing in Living Systems, JACS Au, 2024, 4(2), 384–401.

33. Yuanjiao Yang, Yuru Wang, Zhicong Chao, Yuhui Yang, Yanyun Fang, Ying Liu, Lin Ding, Yunlong Chen*, and Huangxian Ju*, Triply Enhanced Immunotherapy via Dual Glycan Reforming Integrated with Perforation, Advanced Science, 2024, 11(2), 2304971.

************************************************2023***********************************************

32. Yuanjiao Yang#, Shan Wu#, Yunlong Chen*, Huangxian Ju*, Surface-enhanced Raman scattering sensing for detection and mapping of key cellular biomarkers, Chemical Science, 2023, 14(45), 12869–12882.

31. Shiya Zhao#, Yuanjiao Yang#, Yuru Wang, Huipu Liu, Huangxian Ju*, Yunlong Chen*, In situ evaluation of in vivo sialylation with a dual-color imaging strategy, Chemical Communications, 2023, 59(50), 7815–7818.

30. Junjie Hu, Fei Liu, Yunlong Chen, Jia Fu, Huangxian Ju*, Signal-On Mass Spectrometric Biosensing of Multiplex Matrix Metalloproteinases with a Phospholipid-Structured Mass-Encoded Microplate, Analytical Chemistry, 2023, 95(23), 8974–8981.

29. Huipu Liu, Yunlong Chen*, Huangxian Ju*, Functional DNA structures for cytosensing, TRAC-Trends in Analytical Chemistry, 2023, 159, 116933.

28. Yuru Wang, Yuanjiao Yang, Jingxing Guo, Huangxian Ju*, Yunlong Chen*, Tumor identification via in vivo portable Raman detection of sialic acid with dual gold nanoprobe system, Chemical Science, 2023, 14(4), 923–927.

************************************************2022***********************************************


27. Yuanjiao Yang#, Yunlong Chen#, Shiya Zhao, Huipu Liu, Jingxing Guo and Huangxian Ju*, O-GlcNAcylation mapping of single living cells by in situ quantitative SERS imaging, Chemical Science, 2022,13(33), 9701–9705.

26. Yuanjiao Yang (杨媛娇), Yunlong Chen (陈云龙), Huangxian Ju (鞠熀先)*, Progress in surface-enhanced Raman scattering (SERS) imaging of cellular functional molecules and related applications (细胞功能分子的表面增强拉曼成像及其应用研究进展), SCIENTIA SINICA Chimica (中国科学:化学), 2022, 52(9), 1601–1608.

25. Junjie Hu, Fei Liu, Yunlong Chen, Jia Fu, Guoqiang Shangguan, Huangxian Ju*, Mass-Encoded Suspension Array for Multiplex Detection of Matrix Metalloproteinase Activities. Analytical Chemistry, 2022, 94(16), 6380–6386.

24. Yunlong Chen, Yuanjiao Yang, Qingqing Tan, Huipu Liu, Huangxian Ju*, Tumor suppression via diverting intracellular sialylation with multifunctional nanoparticles. Chemical Science, 2022, 13(10), 2939–2945.

************************************************2021***********************************************

23. Junjie Hu, Fei Liu, Yunlong Chen, Guoqiang Shangguan, Huangxian Ju*, Mass Spectrometric Biosensing: A Powerful Approach for Multiplexed Analysis of Clinical Biomolecules. ACS Sensors, 2021, 6(10), 3517–3535.

22. Huipu Liu#, Yunlong Chen#, Jiawei Wang, Yuanjiao Yang, Huangxian Ju*, Tug-of-war: Molecular dynamometers against living cells for analyzing sub-piconewton interaction of specific protein with cell membrane. Chemical Science, 2021, 12(43), 14389–14395.

21. Yuanjiao Yang#, Yunlong Chen#, Jingxing Guo, Huipu Liu, and Huangxian Ju*, A pore-forming protein-induced surface-enhanced Raman spectroscopic strategy for dynamic tracing of cell membrane repair, iScience, 2021, 24(9), 102980.

20. Qiulin Ma#, Yunlong Chen#, Nan Feng, Feng Yan, Huangxian Ju*, A MALDI-MS sensing chip prepared by non-covalent assembly for quantitation of acid phosphatase, Science China-Chemistry, 2021, 64(1), 151–156.

***************************************Before2020***********************************************

19. Yingying Xiong (熊莹莹), Yunlong Chen (陈云龙), Huangxian Ju (鞠熀先)*, Glycan analysis in cellular secretion （细胞分泌物中的聚糖分析）, Acta Chimica Sinica (化学学报) 2019, 77(12), 1221–1229.

18. Yingying Xiong#, Yunlong Chen#, Lin Ding, Xiaoqiang Liu and Huangxian Ju*, Fluorescent visual quantitation of cell-secreted sialoglycoconjugates by chemoselective recognition and hybridization chain reaction, Analyst, 2019, 144, 4545.

17. Jingxing Guo#, Yunlong Chen#, Jianqi Li, Jintong Liu, Huangxian Ju*, A Self-Calibrated 2D Nanoarchitecture for Label-Free SERS Quantitation and Distribution Imaging of Target, Sensors & Actuators: B. Chemical, 2018, 273, 211–219.

16. Jingxing Guo#, Ying Liu#, Yunlong Chen, Jianqi Li, Huangxian Ju*, A Multifunctional SERS Sticky Note for Real-Time Quorum Sensing Tracing and Inactivation of Bacterial Biofilms, Chemical Science, 2018, 9(27), 5906–5911. 

15. Yunlong Chen, Lin Ding, Huangxian Ju*, In Situ Cellular Glycan Analysis, Accounts of Chemical Research, 2018, 51(4), 890–899.

14. Yunlong Chen, Huipu Liu, Lin Ding, Huangxian Ju*, Functional Dual-Color Indicator to Achieve in Situ Visualization of Intracellular Glycosylation, Analytical Chemistry, 2018, 90(5), 3073–3078.

13. Yunlong Chen, Huipu Liu, Yingying Xiong, Huangxian Ju*, Quantitative Screening of Cell-Surface Gangliosides by Nondestructive Extraction and Hydrophobic Collection, Angewandte Chemie-International Edition, 2018, 57(3), 785–789.

12. Jingxing Guo, Yunlong Chen, Yongjia Jiang, Huangxian Ju*, Polyadenine-Modulated DNA Conformation Monitored by Surface-Enhanced Raman Scattering (SERS) on Multibranched Gold Nanoparticles and Its Sensing Application, Chemistry - A European Journal, 2017, 23(39), 9332–9337.

11. Wanyao Song#, Lin Ding#, Yunlong Chen and Huangxian Ju*, Plasmonic Coupling of Dual Gold Nanoprobes for SERS Imaging of Sialic Acids on Living Cells, Chemical Communications, 2016, 52(70), 10640–10643.

10. Yunlong Chen#, Lin Ding#, Wanyao Song, Min Yang, Huangxian Ju*, Liberation of Protein-Specific Glycosylation Information for Glycan Analysis by Exonuclease III-Aided Recycling Hybridization, Analytical Chemistry, 2016, 88(5), 2923–2928.

9. Yunlong Chen#, Lin Ding#, Wanyao Song, Min Yang, Huangxian Ju*, Protein-Specific Raman Imaging of Glycosylation on Single Cells with Zone-Controllable SERS Effect, Chemical Science, 2016, 7(1), 569–574.

8. Yunlong Chen#, Lin Ding#, Junqiang Xu, Wanyao Song, Min Yang, Junjie Hu, Huangxian Ju*, Micro-Competition System for Raman Quantification of Multiple Glycans on Intact Cell Surface, Chemical Science, 2015, 6(7), 3769–3774.

7. Junjie Hu, Rongna Ma, Fei Liu, Yunlong Chen and Huangxian Ju*, Mercaptophenylboronic acid modified gold nanoparticle@silica bubbles for buoyant separation and specific enrichment of glycopeptides, RSC Advances, 2014, 4(55), 28856–28859.

6. Yan Cheng, Jianping Lei*, Yunlong Chen, Huangxian Ju, Highly selective detection of microRNA based on distance-dependent electrochemiluminescence resonance energy transfer between CdTe nanocrystals and Au nanoclusters, Biosensors and Bioelectronics, 2014, 51, 431–436.

5. Yunlong Chen#, Lin Ding#, Tingting Liu, Huangxian Ju*, Arrayed Profiling of Multiple Glycans on Whole Living Cell Surfaces, Analytical Chemistry, 2013, 85(22), 11153–11158.

4. Yunlong Chen, Lin Ding*, Huangxian Ju*, In Situ Tracing of Cell Surface Sialic Acid by Chemoselective Recognition to Unload Gold Nanocluster Probe from Density Tunable Dendrimeric Array, Chemical Communications, 2013, 49(9), 862-864.

3. Lin Ding, Xirui Xiao, Yunlong Chen, Ruocan Qian, Lei Bao and Huangxian Ju*, Competition-based transfer of carbohydrate expression information from a cell-adhered surface to a secondary surface, Chemical Communications, 2011, 47(13), 3742–3744.

2. Wei Cheng, Yunlong Chen, Feng Yan, Lin Ding, Shijia Ding, Huangxian Ju* and Yibing Yin*, Ultrasensitive scanometric strategy for detection of matrix metalloproteinases using a histidine tagged peptide-Au nanoparticle probe, Chemical Communications, 2011, 47(10), 2877–2879.

1. Wei Cheng, Lin Ding, Yunlong Chen, Feng Yan, Huangxian Ju* and Yibing Yin*, A facile scanometric strategy for ultrasensitive detection of protein using aptamer-initiated rolling circle amplification, Chemical Communications, 2010, 46(36), 6720–6722.

Course Name, Time and Place

Modern instrumental analysis; Instrumental analysis experiments

Syllabus, Examination Requirements
Teaching Resources
Group

Please visit https://sklac.nju.edu.cn/hxju_en/