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    发布时间:2018-11-21 10:44:53    点击:676

学术报告


题  目:Synthetic DNA Molecules with Catalytic and Binding Properties as
Sensors
报告人:Yingfu Li教授
单  位: Department of Biochemistry and Biomedical Sciences
        Department of Chemistry and Chemical Biology
        School of Biomedical Engineering
        Michael G. DeGroote Institute of Infectious Disease Research,
        Biointerfaces Institute McMaster University, Canada
地  点:化学楼H201蒋雯若报告厅
时  间:2018年11月29日(星期四)上午10:00
联系人:刘震 教授
 
 
摘要:
Our laboratory has been interested in developing simple biosensing devices or assays that use two classes of synthetic DNA molecules: DNA aptamers (DNA-based molecular receptors) and DNAzymes (catalytic DNAs). For example, we have successfully developed DNAzymes that are capable of tracking Escherichia coli (a common water/food-borne pathogen) and Clostridium difficile (a common cause for healthcare-associated infections in North America and Europe), and currently we are working on developing similar DNAzymes for other important human pathogens. Furthermore, given the fact that DNA is a highly programmable material based on predictable Watson–Crick basepairing interactions, we have been interested in engineering molecular devices in which a key player is a DNA aptamer or ligand-responsive DNAzyme to achieve ultra-sensitive target detection. For example, we have developed several simple biosensing DNA devices/assays built with various linear and circular aptamer/DNAzyme-containing complexes that can be activated by a target of interest. In this presentation, I will discuss the progress we have made and challenges we have encountered from these efforts.
 
 
简历:
Yingfu Li was born and raised in Anhui, China. He received his BSc in chemistry at Anhui University in 1983, and his MSc in applied chemistry at Beijing Agriculture University in 1989. He moved to Canada in 1992, and in 1997, he graduated with a PhD in chemistry and biochemistry at Simon Fraser University under the supervision of Professor Dipankar Sen. His doctoral study led to the discovery of one of the earliest DNA molecules with catalytic ability using a powerful test-tube evolution technique known as in vitro selection. This work won him Governor General of Canada Gold Medal (1998) and a Doctoral Prize from the Natural Sciences and Engineering Research Council of Canada (1998). He received a Medical Research Council of Canada postdoctoral fellow in 1997 and spent the next two years in the laboratory of Ronald Breaker at Yale University to study artificial evolution using DNA as the model system. Through test-tube evolution he derived a series of nucleic acid enzymes that are capable of modifying DNA using chemical reactions that are carried out in cells by protein enzymes. In 1999, he joined McMaster University as an assistant professor in the Department of Biochemistry and Biomedical Sciences and the Department of Chemistry and Chemical Biology, was promoted to the rank of associate professor in 2005 and full professor in 2010. At McMaster, he has established a research group to take advantage of the powerful test-tube evolution technique to develop artificial DNA and RNA molecules that can be used to address fundamental biochemical and evolutionary questions or can be utilized for wide-ranging applications. He has published extensively in the fields of chemistry, biochemistry and molecular evolution of nucleic acids. He has published ~180 research and review articles, ~20 book chapters, edited 1 book, and filed over 20 patents. He also serves as an associate editor of Journal of Molecular Evolution and as a member of editorial board of Scientific Reports. He has received several recognitions, including Canada Research Chair, New Investigator Award from the Canadian Institute of Health Research (CIHR), Premier Research Excellent Award from Ontario Government, and W. A. McBryde Medal from Canadian Society of Chemistry.  
 
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