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    发布时间:2017-07-04 10:12:38    点击:872

学术报告


报告题目:Single-molecule chemistry: from solar energy conversion to transcription regulation

报告人:陈鹏 (Peter Debye Professor of Chemistry, Cornell University)

报告时间:2017年7月7日(周五)上午10:00

报告地点:仙林化学楼H201报告厅

联系人:王伟

报告内容简介:
    I will present two stories that use single-molecule fluorescence imaging to interrogate the mechanism and dynamics of chemical and biological processes. In the first story, I will present our work in using redox-selective super-resolution reaction imaging and sub-particle photocurrent measurements to determine the relation between charge-carrier surface activity and water oxidation efficiency on a semiconductor photoanode during photoelectrochemical water oxidation. We determine that on a semiconductor nanostructure, how much the sites reactive for water oxidation also contribute to recombination, and how the photocurrent efficiency and onset potential are correlated at the nanoscale. Guided by hole and electron reactivity maps, site-selective catalyst deposition on individual nanorods reveals the optimal sites for oxygen evolution catalysts, leading to a strategy for rationally engineering photoelectrodes with catalysts. In the second story, I will present our work in using super-resolution single-molecule tracking to study transcription regulation in living cells. Binding and unbinding of transcription regulators on chromosome constitute a primary mechanism for gene regulation. While many cellular factors are known to regulate their binding, little is known on how cells can modulate their unbinding for regulation. I will describe that two metal-sensing transcription regulators show unusual concentration and chromosome-conformation dependent unbinding kinetics in bacterial cells, which provide novel mechanisms for facile switching between transcription activation and deactivation in vivo and in coordinating transcription regulation of resistance genes with the cell cycle.

Key references:
1. J. B. Sambur, T.-Y. Chen, E. Choudhary, G. Chen, E. J. Nissen, E. M. Thomas, N. Zou, P. Chen "Sub-particle reaction and photocurrent mapping to optimize catalyst-modified photoanodes" Nature 2016, 530, 77-80.
2. X. Zhou, N. M. Andoy, G. Liu, E. Choudhary, K.-S. Han, H. Shen, P. Chen "Quantitative super-resolution imaging uncovers reactivity patterns on single nanocatalysts" Nature Nanotech. 2012, 7, 237–241.
3. T.-Y. Chen, A. G. Santiago, W. Jung, L. Krzeminski, F. Yang, D. J. Martell, J. D. Helmann, P. Chen “Concentration and chromosome-organization dependent regulator unbinding from DNA for transcription regulation in living cells” Nature Commun. 2015, 6, 7445. DOI: 10.1038/ncomms8445. 
4. C. P. Joshi, D. Panda, D. J. Martell, N. M. Andoy, T.-Y. Chen, A. Gaballa, J. D. Helmann, P. Chen "Direct substitution and assisted dissociation pathways for turning off transcription by a MerR-family metalloregulator" Proc. Natl. Acad. Sci. USA 2012, 109, 15121-15126.

报告人简介:
    Peng Chen is the Peter Debye Professor of Chemistry at Cornell University. He received his B.S. in Chemistry from Nanjing University, China in 1997. After a year at University of California, San Diego with Prof. Yitzhak Tor learning organic synthesis, he moved to Stanford University and did his Ph.D. with Prof. Edward Solomon in bioinorganic/physical inorganic chemistry. In January 2004, he joined Prof. Sunney Xie’s group at Harvard University for postdoctoral research in single-molecule biophysics. He started his faculty appointment at Cornell University in July 2005. His current research focuses on single-molecule imaging of nanoscale (photo)(electro)catalysis, as well as of metal homeostatic machineries in vitro and in living cells. He has received Dreyfus New Faculty Award, NSF Career Award, Sloan Fellowship, Paul Saltman Award, CAPA Distinguished Junior Faculty Award, Coblentz Award, ACS Phys Division Early-Career Award in Experimental Physical Chemistry, and Excellence in Catalysis Award from the Catalysis Society of Metro NY, etc.

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